- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-13914m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
SWI
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse SWI/SNF complex subunit SMARCC1, Smarcc1 ELISA KIT
Product Name:Mouse SWI/SNF complex subunit SMARCC1, Smarcc1 ELISA KIT
Packing:96T
Catalog No.:ELI-13914m
Gene Name:Mouse Smarcc1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Smarcc1
Alternative Name:Mouse SWI/SNF complex subunit SMARCC1, Smarcc1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse SWI/SNF complex subunit SMARCC1, Smarcc1 ELISA KIT allows for the in vitro quantitative determination of Mouse Smarcc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse SWI/SNF complex subunit SMARCC1, Smarcc1 ELISA KIT has been pre-coated with an Mouse SWI/SNF complex subunit SMARCC1, Smarcc1 antibody specific to Mouse Smarcc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Smarcc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Smarcc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Smarcc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验三句话读懂一篇 CNS：曹雪涛团队最新研究揭示免疫炎症调控新机制；每天多运动 20 分钟，可降低 9 种相关疾病风险
：曹雪涛院士团队发现泛素化修饰调控免疫炎症的新机制 目前，SMARCC1 调控炎症先天反应的机制尚不清楚。 2023 年 2 月 15 日，中国医学科学院北京协和医学院曹雪涛及姜明红共同通讯在 Cell Reports 杂志发表研究论文 RNF138 inhibits late inflammatory gene transcription through degradation of SMARCC1 of the SWI/SNF complex。 该研究发现核 E3 泛素连接酶 RNF
系统照相。 超声波裂解细菌 渗透休克细菌沉淀加1/10培养体积20 mM Tris-HCl（pH 7.5，0℃）重悬，冰浴，超声波裂解，20%工作选择，脉冲粉碎1 min，然后13000 r/min离心5 min，-20℃保存上清、细菌沉淀。上清进行TCA沉淀，处理同上。沉淀用Protein Extraction Reagent（Ni-NTAHi Bind Purification Kit BugBuster. 提供 ）按 Novagen公司《pET System Manual》提供方法反复处理
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