Coenzyme Q-binding protein COQ
10 homolog B mitochondrial, / Mouse Coenzyme Q-binding protein COQ10 homolog B mitochondrial,- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-06656m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Coenzyme Q-binding protein COQ10 homolog B mitochondrial, 含量
- 适应物种：
小鼠
- 标记物：
Coenzyme Q-binding protein COQ10 homolog B mitochondrial,
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Coenzyme Q-binding protein COQ10 homolog B mitochondrial, CQ10B ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
COQ10B,coenzyme Q-binding protein COQ10 homolog B mitochondrial,coenzyme Q10 homolog B
Search name
Mouse COQ10B ELISA KIT ,Mouse coenzyme Q-binding protein COQ10 homolog B mitochondrial ELISA KIT ,Mouse coenzyme Q10 homolog B ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse coenzyme q-binding protein coq10 homolog b, mitochondrial,CQ10B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CQ10B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CQ10B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CQ10B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CQ10B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Assay to Measure Oxidized and Reduced Forms of CoQ by LCMS/MS
The redox status of mitochondrial coenzyme Q (CoQ) is an important marker for oxidative stress associated with several disorders such as Parkinson disease and Alzheimer disease. Altered redox status may be present in mitochondrial electron
solution (10% (wt/vol) TCA). (8)Sonicate cells for 30 s on ice at a rate of 1 pulse per s. (9) Spin the sonicated cell lysate at 15,000g for 5 min at 4 °C to precipitate the protein pellet. (10)SPE (Steps 10–16). Using a vacuum
Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
The human mitochondrial genome. The structural genes for the mtDNA-encoded 12S and 16S ribosomal RNAs, the subunits of NADH-coenzyme Q oxidoreductase (ND), cytochrome-c oxidase (COX), cytochrome-b (Cyt b), and ATP synthase (A), and 22 tRNAs, are shown
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