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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Kinetochore protein Nuf2, Nuf2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Kinetochore protein Nuf2, Nuf2 ELISA KIT
Product Name:Mouse Kinetochore protein Nuf2, Nuf2 ELISA KIT
Packing:96T
Catalog No.:ELI-13772m
Gene Name:Mouse Nuf2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Nuf2
Alternative Name:Mouse Kinetochore protein Nuf2, Nuf2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Kinetochore protein Nuf2, Nuf2 ELISA KIT allows for the in vitro quantitative determination of Mouse Nuf2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Kinetochore protein Nuf2, Nuf2 ELISA KIT has been pre-coated with an Mouse Kinetochore protein Nuf2, Nuf2 antibody specific to Mouse Nuf2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nuf2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nuf2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nuf2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
_003380 1037-1059 aaatggaagagaactttgccg Human HeLa vinculin, mouse (1) L18880 2923-2945 aagcggattagaaccaatctc Mouse 3T3 zyxin, mouse (1) X99063 1357-1379 aaagcctaccacccacagtgc Mouse 3T3 参考文献 Harborth J, Elbashir SM, Bechert K, Tuschl T
_003380 1037-1059 aaatggaagagaactttgccg Human HeLa View, Order View vinculin, mouse (1) L18880 2923-2945 aagcggattagaaccaatctc Mouse 3T3 View, Order View zyxin, mouse (1) X99063
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