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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Transmembrane protein 179, Tmem179
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transmembrane protein 179, Tmem179 ELISA KIT
Product Name:Mouse Transmembrane protein 179, Tmem179 ELISA KIT
Packing:96T
Catalog No.:ELI-13711m
Gene Name:Mouse Tmem179
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Tmem179
Alternative Name:Mouse Transmembrane protein 179, Tmem179
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transmembrane protein 179, Tmem179 ELISA KIT allows for the in vitro quantitative determination of Mouse Tmem179 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transmembrane protein 179, Tmem179 ELISA KIT has been pre-coated with an Mouse Transmembrane protein 179, Tmem179 antibody specific to Mouse Tmem179 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tmem179 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tmem179 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tmem179 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Basic Protein Sequence Analysis
for Understanding Results of Predictions of Helical Transmembrane Regions and Subcellular Localization Alternate Protocol 3: Predicting the Subcellular Localization of a Protein Using Targetp Support Protocol 5: Guidelines
Semiquantitative immunoblots of membrane protein-epidermal growth factor
Membrane proteins or cytokines are sometimes difficult to isolate and purify. Our group recently concentrated on epidermal growth factor (EGF) protein expression studies. Mature EGF was initially identified from mouse submaxillary gland extract
Synchronizing Protein Transport in the Secretory Pathway
, Interleukin‐2 signal sequence; SBP, streptavidin‐binding peptide; X, protein of interest. View Image
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