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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
IQ domain-containing protein H, Iqch
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse IQ domain- containing protein H, Iqch ELISA KIT
Product Name:Mouse IQ domain- containing protein H, Iqch ELISA KIT
Packing:96T
Catalog No.:ELI-13552m
Gene Name:Mouse Iqch
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Iqch
Alternative Name:Mouse IQ domain-containing protein H, Iqch
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse IQ domain- containing protein H, Iqch ELISA KIT allows for the in vitro quantitative determination of Mouse Iqch concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse IQ domain- containing protein H, Iqch ELISA KIT has been pre-coated with an Mouse IQ domain-containing protein H, Iqch antibody specific to Mouse Iqch .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Iqch and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Iqch , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Iqch in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DNA Extraction Promega DNA-IQ System Database Protocol
piece of bench paper over the area in which you will be working and then make sure you have all of the following on the work table: Your personal DNA extraction kit Box of gloves (your size) A box of filter tips (small size
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Immunostaining 24. Wash stored slides twice in PBS (10 min each wash). 25. Wash slides once in PBS containing 1% Triton X-100 for 10 min. 26. Incubate slides in a slide jar containing blocking solution for 1 h
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of 0.3% SDS). Mix by gentle pipetting. Shake gently for 1 h at 37°C. 15. Add 50 µL of 20% Triton X-100 (to a final concentration of 1.8%). Shake gently for 1 h at 37°C. 16. Add 1200 units of restriction enzyme (i.e., 12 µL
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