Scaffold attachment factor B1,

Mouse Scaffold attachment factor B1, Safb ELISA KIT

Product Name:Mouse Scaffold attachment factor B1, Safb ELISA KIT
Packing:96T

Catalog No.:ELI-13500m

Gene Name:Mouse Safb

Detect Range:78.125-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Safb

Alternative Name:Mouse Scaffold attachment factor B1, Safb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Scaffold attachment factor B1, Safb ELISA KIT allows for the in vitro quantitative determination of Mouse Safb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Scaffold attachment factor B1, Safb ELISA KIT has been pre-coated with an Mouse Scaffold attachment factor B1, Safb antibody specific to Mouse Safb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Safb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Safb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Safb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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