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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Serine protease inhibitor Kazal-type 7, Spink7
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Serine protease inhibitor Kazal- type 7, Spink7 ELISA KIT
Product Name:Mouse Serine protease inhibitor Kazal- type 7, Spink7 ELISA KIT
Packing:96T
Catalog No.:ELI-12850m
Gene Name:Mouse Spink7
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Spink7
Alternative Name:Mouse Serine protease inhibitor Kazal-type 7, Spink7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine protease inhibitor Kazal- type 7, Spink7 ELISA KIT allows for the in vitro quantitative determination of Mouse Spink7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Serine protease inhibitor Kazal- type 7, Spink7 ELISA KIT has been pre-coated with an Mouse Serine protease inhibitor Kazal-type 7, Spink7 antibody specific to Mouse Spink7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Spink7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Spink7 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Spink7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验) or early death. The serine protease uPA, in concert with its inhibitor PAI-1, promotes tumor cell adhesion, migration, and proliferation, as well as extracellular matrix degradation and, thus, facilitates tumor cell invasion and metastasis. The various
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
receptor cytoplasmic domainI[235]Interleukin-17 (hIL-17)S, 0.35 mg/L, α-MF[236]Intracellular proteinase inhibitor (PI-6)I, 50 mg/L[237]Intrinsic factorS, 40 mg/L, α-MF, functional447Kunitz-type protease inhibitor domain of protease nexin-2/Amyloid b-protein
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