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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Serine protease inhibitor Kazal-type 2, Spink2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Spink2 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse Spink2 ELISA KIT ,Mouse HUSI II ELISA KIT ,Mouse HUSI-II ELISA KIT ,Mouse serine protease inhibitor Kazal-type 2 ELISA KIT ,Mouse epididymis tissue protein Li 172 ELISA KIT ,Mouse serine peptidase inhibitor ELISA KIT ,Mouse Kazal type 2 (acrosin-trypsin inhibitor) ELISA KIT ,Mouse serine peptidase inhibitor Kazal type 2 ELISA KIT
Intended use
Mouse Spink2 ELISA kit allows for the in vitro quantitative determination of Mouse Spink2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse Spink2 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Spink2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Spink2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Spink2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Spink2 ELISA kit Range 0.15-10ng/ml
Spink2 Related Products
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Rat Serine protease inhibitor Kazal- type 2, Spink2 ELISA KIT
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: 3128136151
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文献和实验) or early death. The serine protease uPA, in concert with its inhibitor PAI-1, promotes tumor cell adhesion, migration, and proliferation, as well as extracellular matrix degradation and, thus, facilitates tumor cell invasion and metastasis. The various
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
Phosphate‐buffered saline (PBS; Sigma, cat. no. D8537) Nuclear extract buffer A (see recipe ) Protease inhibitor
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