DNA repair protein RAD51 homol
og 2, Rad51b ELISA KIT/ Mouse DNA repair protein RAD51 homolog 2, Rad51b ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22146m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
DNA repair protein RAD51 homolog 2, Rad51b
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse DNA repair protein RAD51 homolog 2, Rad51b ELISA KIT
Product Name:Mouse DNA repair protein RAD51 homolog 2, Rad51b ELISA KIT
Packing:96T
Catalog No.:ELI-22146m
Gene Name:Mouse Rad51b
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Rad51b
Alternative Name:Mouse DNA repair protein RAD51 homolog 2, Rad51b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA repair protein RAD51 homolog 2, Rad51b ELISA KIT allows for the in vitro quantitative determination of Mouse Rad51b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA repair protein RAD51 homolog 2, Rad51b ELISA KIT has been pre-coated with an Mouse DNA repair protein RAD51 homolog 2, Rad51b antibody specific to Mouse Rad51b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rad51b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rad51b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rad51b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
。 Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5120 Assay for Human Rad51-Mediated DNA Displacement Loop Formation Steven Raynard and Patrick Sung1 Department of Molecular Biophysics and Biochemistry, Yale
Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Rad51-mediated pairing between homologous DNA sequences during homologous recombination (HR) plays pivotal roles in DNA double-strand break repair. The multi-step process occurs through cooperation of Rad51 and a number of accessory protein
In Vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
/Rad51 family of proteins are the key enzymes in this homology-directed repair pathway, as they perform DNA strand invasion and exchange, in concert with a host of ancillary factors. In vitro, the RecA/Rad51 family of proteins share similar enzymatic
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