A-kinase anchor protein 8, Aka
p8 ELISA KIT/ Mouse A-kinase anchor protein 8, Akap8 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-11941m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
A-kinase anchor protein 8, Akap8
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse A- kinase anchor protein 8, Akap8 ELISA KIT
Product Name:Mouse A- kinase anchor protein 8, Akap8 ELISA KIT
Packing:96T
Catalog No.:ELI-11941m
Gene Name:Mouse Akap8
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Akap8
Alternative Name:Mouse A-kinase anchor protein 8, Akap8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse A- kinase anchor protein 8, Akap8 ELISA KIT allows for the in vitro quantitative determination of Mouse Akap8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse A- kinase anchor protein 8, Akap8 ELISA KIT has been pre-coated with an Mouse A-kinase anchor protein 8, Akap8 antibody specific to Mouse Akap8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Akap8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Akap8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Akap8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
proteins) are relatively difficult to investigate by standard yeast two?hybrid methods either because they are retained at cellular membranes or they activate the system in the absence of a true protein interaction. The membrane?based yeast two?hybrid (MbY
, a protein–membrane binding detection assay is described through the example of protein kinase Cα binding to surface-immobilized whole liposomes (see Subheading 3.4).
protein. Twelve chicken GPI-anchor proteins: AXO1_CHICK Axonin-1 precursor: 1036 aa P28685 EPL6_CHICK EPH-related receptor tyrosine kinase ligand 6 precursor (lerk-6)200 aa P52802 G55A_CHICK Neurite inhibitor gp55a (fragment) 274 aa Q98892 GLYP
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