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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白ADAM DEC1, Adamdec1含量
- 适应物种:
小鼠
- 标记物:
ADAM DEC1, Adamdec1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse ADAM DEC1, Adamdec1 ELISA KIT
Product Name:Mouse ADAM DEC1, Adamdec1 ELISA KIT
Packing:96T
Catalog No.:ELI-11570m
Gene Name:Mouse Adamdec1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Adamdec1
Alternative Name:Mouse ADAM DEC1, Adamdec1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse ADAM DEC1, Adamdec1 ELISA KIT allows for the in vitro quantitative determination of Mouse Adamdec1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse ADAM DEC1, Adamdec1 ELISA KIT has been pre-coated with an Mouse ADAM DEC1, Adamdec1 antibody specific to Mouse Adamdec1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Adamdec1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Adamdec1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Adamdec1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验一点小东西希望对大家有些用处 We improved this resource This page was translated from french to it is now finalized Thank you for any comment IMMUNOLOGICAL TECHNIQUES Tools and Techniques Molecular Immunology Antibodies. Antibody uses: Elisa
Intracellular Cytokine Staining Protocol
.In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines, it is necessary to block secretion of cytokines with reagents such as Monensin or Brefeldin A during the last few hours of the stimulation. It is advised
15 August 2011. ^ a b Wheeler, B.; Heimberg, A.; Moy, V.; Sperling, E.; Holstein, T.; Heber, S.; Peterson, K. (2009). "The deep evolution of metazoan microRNAs". Evolution & development 11 (1): 50–68. doi:10.1111/j.1525-142X.2008.00302.x. PMID
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