B2 bradykinin receptor, Bdkrb2

Mouse B2 bradykinin receptor, BDKRB2 ELISA Kit (CUSTOM)

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse BDKRB2 ELISA KIT ,Mouse B2R ELISA KIT ,Mouse BK-2 ELISA KIT ,Mouse BK2 ELISA KIT ,Mouse BKR2 ELISA KIT ,Mouse BRB2 ELISA KIT ,Mouse BK-2 receptor ELISA KIT ,Mouse bradykinin receptor B2 ELISA KIT

Intended use

Mouse BDKRB2 ELISA kit allows for the in vitro quantitative determination of Mouse B2 bradykinin receptor concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse BDKRB2 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BDKRB2, During the reaction, BDKRB2 in the sample or standard competes with a fixed amount of biotin-labeled BDKRB2 for sites on a pre-coated Monoclonal antibody specific to BDKRB2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BDKRB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.