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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白CDK5 regulatory subunit-associated protein 2, Cdk5rap2 EL含量
- 适应物种:
小鼠
- 标记物:
CDK5 regulatory subunit-associated protein 2, Cdk5rap2 EL
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse CDK5 regulatory subunit- associated protein 2, CDK5RAP2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDK5RAP2,C48; Cep215; MCPH3; CDK5 activator-binding protein C48; CDK5 regulatory subunit-associated protein 2; centrosomal protein 215 kDa; centrosomin; CDK5 regulatory subunit associated protein 2
Search name
Mouse CDK5RAP2 ELISA KIT ,Mouse C48 ELISA KIT ,Mouse Cep215 ELISA KIT ,Mouse MCPH3 ELISA KIT ,Mouse CDK5 activator-binding protein C48 ELISA KIT ,Mouse CDK5 regulatory subunit-associated protein 2 ELISA KIT ,Mouse centrosomal protein 215 kDa ELISA KIT ,Mouse centrosomin ELISA KIT ,Mouse CDK5 regulatory subunit associated protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse CDK5 regulatory subunit- associated protein 2, CDK5RAP2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDK5 regulatory subunit-associated protein 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CDK5 regulatory subunit-associated protein 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CDK5 regulatory subunit-associated protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CDK5 regulatory subunit-associated protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验Cyclin-Dependent Kinase 5 (Cdk5): Preparation and Measurement of Kinase Activity
and to elucidate the mechanism regulating its activity. Cdk5 is a catalytic subunit of an active kinase complex with the regulatory subunit, p35 or p39. Because the kinase activity of Cdk5 is regulated mainly by synthesis and degradation
Overview of Protein Microarrays
samples. Anal. Biochem. 414:99‐102. Guo, A. and Zhu, X.‐Y. 2006. The critical role of surface chemistry in protein microarrays. In Functional Protein Microarrays in Drug
Characterization and Validation of Cre‐Driver Mouse Lines
Mol. Biol. 530:1‐14. Pinto, D., Robine, S., Jaisser, F., El Marjou, F.E., and Louvard, D. 1999. Regulatory sequences of the mouse villin gene that efficiently drive
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