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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Pre-mRNA-splicing factor CWC25 homolog, Cwc25含量
- 适应物种:
小鼠
- 标记物:
Pre-mRNA-splicing factor CWC25 homolog, Cwc25
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Pre- mRNA- splicing factor CWC25 homolog, Cwc25 ELISA KIT
Product Name:Mouse Pre- mRNA- splicing factor CWC25 homolog, Cwc25 ELISA KIT
Packing:96T
Catalog No.:ELI-10256m
Gene Name:Mouse Cwc25
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cwc25
Alternative Name:Mouse Pre-mRNA-splicing factor CWC25 homolog, Cwc25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Pre- mRNA- splicing factor CWC25 homolog, Cwc25 ELISA KIT allows for the in vitro quantitative determination of Mouse Cwc25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Pre- mRNA- splicing factor CWC25 homolog, Cwc25 ELISA KIT has been pre-coated with an Mouse Pre-mRNA-splicing factor CWC25 homolog, Cwc25 antibody specific to Mouse Cwc25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cwc25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cwc25 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cwc25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
NM_005381 Homo sapiens nucleolin (NCL), mRNA 2043 NM_005439 Homo sapiens myeloid leukemia factor 2 (MLF2), mRNA 697 NM_006445 Homo sapiens PRP8 pre-mRNA processing factor 8 homolog (yeast) (PRPF8), mRNA 1384 NM_019059 Homo sapiens homolog
所有的看家基因(housekeeping genes)的列表+引物设计服务
697 NM_006445 Homo sapiens PRP8 pre-mRNA processing factor 8 homolog (yeast) (PRPF8), mRNA 1384 NM_019059 Homo sapiens homolog of Tom7 (S. cerevisiae) (TOM7), mRNA 2364 NM_006039
。两者主要的区别在于起源上: miRNA 是内源性的,从编码miRNA 的基因转录长链初始miRNA (pri-miRNA),分别在核内和胞浆经两步加工形成;siRNA 则是从内源或外源的dsRNA 前体中生成,在胞浆内加工成熟,不需要Drosha 等细胞核内因子参与。此外,siRNA引发mRNA的降解,而动物miRNA 主要抑制mRNA 的翻译。 1.1 Drosha 及其辅助蛋白质 Drosha 负责pr imiRNA核内加工,属于RNase III 家族第II 亚家族中的一种酶
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