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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白DCC-interacting protein 13-alpha, Appl1含量
- 适应物种:
小鼠
- 标记物:
DCC-interacting protein 13-alpha, Appl1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DCC- interacting protein 13- alpha, Appl1 ELISA KIT
Product Name:Mouse DCC- interacting protein 13- alpha, Appl1 ELISA KIT
Packing:96T
Catalog No.:ELI-09667m
Gene Name:Mouse Appl1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Appl1
Alternative Name:Mouse DCC-interacting protein 13-alpha, Appl1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DCC- interacting protein 13- alpha, Appl1 ELISA KIT allows for the in vitro quantitative determination of Mouse Appl1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DCC- interacting protein 13- alpha, Appl1 ELISA KIT has been pre-coated with an Mouse DCC-interacting protein 13-alpha, Appl1 antibody specific to Mouse Appl1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Appl1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Appl1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Appl1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
ATGTTAAGACTAAGCATGTAC Xenopus embryo cytoplasmic dynein 1 heavy chain (1) U53530 509-531 aaggccaaggaggcgctggaa Human HeLa DIP13-alpha (DCC interacting protein) (25) AAGUGCAGCAACACACCUGAC DLD1 DIP13-beta (DCC interacting protein) (25) AAGCAGCACCUCUCCUCCCUU DLD
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