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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Laminin subunit gamma-2, Lamc2含量
- 适应物种:
小鼠
- 标记物:
Laminin subunit gamma-2, Lamc2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Laminin subunit gamma- 2, Lamc2 ELISA KIT
Product Name:Mouse Laminin subunit gamma- 2, Lamc2 ELISA KIT
Packing:96T
Catalog No.:ELI-04816m
Gene Name:Mouse Lamc2
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Lamc2
Alternative Name:Mouse Laminin subunit gamma-2, Lamc2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Laminin subunit gamma- 2, Lamc2 ELISA KIT allows for the in vitro quantitative determination of Mouse Lamc2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Laminin subunit gamma- 2, Lamc2 ELISA KIT has been pre-coated with an Mouse Laminin subunit gamma-2, Lamc2 antibody specific to Mouse Lamc2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lamc2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lamc2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lamc2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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with the recommended amount of ATP, 2 μl of gamma-33P-ATP (20 μCi), and 2 μl of purified enzyme (10 units of cAMP-dependent protein kinase (catalytic subunit), 1000 units of casein kinase II, or 100 units of Erk2). Following the 1 hr incubation, the slides were washed
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