- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-09011m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Cytochrome b5 type B, Cyb5b含量
- 适应物种：
小鼠
- 标记物：
Cytochrome b5 type B, Cyb5b
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Cytochrome b5 type B, Cyb5b ELISA KIT
Product Name:Mouse Cytochrome b5 type B, Cyb5b ELISA KIT
Packing:96T
Catalog No.:ELI-09011m
Gene Name:Mouse Cyb5b
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Cyb5b
Alternative Name:Mouse Cytochrome b5 type B, Cyb5b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytochrome b5 type B, Cyb5b ELISA KIT allows for the in vitro quantitative determination of Mouse Cyb5b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cytochrome b5 type B, Cyb5b ELISA KIT has been pre-coated with an Mouse Cytochrome b5 type B, Cyb5b antibody specific to Mouse Cyb5b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cyb5b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cyb5b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cyb5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
by PAGE usingthe following formula: Indel percentage (%) = (1 . √(1 . x )) × 100, where x = ( a + b )/( a + b + c ) In this formula, the number “ a ” and “ b ” represent therelative quantities of the cleaved bands, while “ c ” equals
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cytochrome c in fixed cells. The Alexa Fluor 488 dye exhibits bright green fluorescence that is compatible with filters and instrument settings appropriate for fluorescein. Each kit contains: Mouse IgG1 anti–cytochrome c antibody Highly cross-adsorbed Alexa
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