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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Early endosome antigen 1, Eea1含量
- 适应物种:
小鼠
- 标记物:
Early endosome antigen 1, Eea1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Early endosome antigen 1, EEA1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
EEA1,early endosome antigen 1; early endosome antigen 1, 162kD; MST105; MSTP105; ZFYVE2; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2
Search name
Mouse EEA1 ELISA KIT ,Mouse early endosome antigen 1 ELISA KIT ,Mouse early endosome antigen 1 ELISA KIT ,Mouse 162kD ELISA KIT ,Mouse MST105 ELISA KIT ,Mouse MSTP105 ELISA KIT ,Mouse ZFYVE2 ELISA KIT ,Mouse early endosome-associated protein ELISA KIT ,Mouse endosome-associated protein p162 ELISA KIT ,Mouse zinc finger FYVE domain-containing protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Early endosome antigen 1concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toEarly endosome antigen 1, During the reaction,Early endosome antigen 1in the sample or standard competes with a fixed amount of biotin-labeledEarly endosome antigen 1 for sites on a pre-coated Monoclonal antibody specific to Early endosome antigen 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Early endosome antigen 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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