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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Interleukin-3 receptor subunit alpha, Il3ra含量
- 适应物种:
小鼠
- 标记物:
Interleukin-3 receptor subunit alpha, Il3ra
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Interleukin-3 receptor subunit alpha, IL3ra ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Interleukin-3 receptor subunit alpha,IL-3 receptor subunit alpha,IL-3R subunit alpha,IL-3R-alpha,IL-3RA,CD123,interleukin-3 receptor subunit alpha; IL3R; IL3RAY; IL3RX; IL3RY; hIL-3Ra; CD123 antigen; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; interleukin 3 receptor alpha (low affinity)
Search name
Mouse Interleukin-3 receptor subunit alpha ELISA KIT ,Mouse IL-3 receptor subunit alpha ELISA KIT ,Mouse IL-3R subunit alpha ELISA KIT ,Mouse IL-3R-alpha ELISA KIT ,Mouse IL-3RA ELISA KIT ,Mouse CD123 ELISA KIT ,Mouse interleukin-3 receptor subunit alpha ELISA KIT ,Mouse IL3R ELISA KIT ,Mouse IL3RAY ELISA KIT ,Mouse IL3RX ELISA KIT ,Mouse IL3RY ELISA KIT ,Mouse hIL-3Ra ELISA KIT ,Mouse CD123 antigen ELISA KIT ,Mouse IL-3 receptor alpha SP2 isoform ELISA KIT ,Mouse IL-3 receptor subunit alpha ELISA KIT ,Mouse IL-3R subunit alpha ELISA KIT ,Mouse IL-3R-alpha ELISA KIT ,Mouse IL-3RA ELISA KIT ,Mouse interleukin 3 receptor alpha (low affinity) ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse interleukin-3 receptor subunit alpha,IL-3 receptor subunit alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-3 receptor subunit alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-3 receptor subunit alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-3 receptor subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-3 receptor subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
. Activation of the lung S1P1 receptor reduces allergen‐induced plasma leakage in mouse. Brit. J. Pharmacol. 158:1295‐1301. Delayre‐Orthez, C., Becker, J., De Blay, F., Frossard, N
such as neuronal differentiation and bone loss. To produce these effects IL-6 signals through a receptor composed of two different subunits, an alpha subunit that produces ligand specificity and gp130, a receptor subunit shared in common with other cytokines
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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