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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Lysine-specific demethylase 6B, Kdm6b含量
- 适应物种:
小鼠
- 标记物:
Lysine-specific demethylase 6B, Kdm6b
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Lysine- specific demethylase 6B, KDM6B ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Kdm6b,JMJD3; jmjC domain-containing protein 3; jumonji domain containing 3, histone lysine demethylase; jumonji domain-containing protein 3; lysine demethylase 6B; lysine-specific demethylase 6B; lysine (K)-specific demethylase 6B
Search name
Mouse Kdm6b ELISA KIT ,Mouse JMJD3 ELISA KIT ,Mouse jmjC domain-containing protein 3 ELISA KIT ,Mouse jumonji domain containing 3 ELISA KIT ,Mouse histone lysine demethylase ELISA KIT ,Mouse jumonji domain-containing protein 3 ELISA KIT ,Mouse lysine demethylase 6B ELISA KIT ,Mouse lysine-specific demethylase 6B ELISA KIT ,Mouse lysine (K)-specific demethylase 6B ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Lysine- specific demethylase 6B, KDM6B concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysine- specific demethylase 6B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Lysine- specific demethylase 6B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Lysine- specific demethylase 6B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Lysine- specific demethylase 6B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
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