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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Guanylyl cyclase-activating protein 1, Guca1a含量
- 适应物种:
小鼠
- 标记物:
Guanylyl cyclase-activating protein 1, Guca1a
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Guanylyl cyclase- activating protein 1, Guca1a ELISA KIT
Product Name:Mouse Guanylyl cyclase- activating protein 1, Guca1a ELISA KIT
Packing:96T
Catalog No.:ELI-08473m
Gene Name:Mouse Guca1a
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Guca1a
Alternative Name:Mouse Guanylyl cyclase-activating protein 1, Guca1a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Guanylyl cyclase- activating protein 1, Guca1a ELISA KIT allows for the in vitro quantitative determination of Mouse Guca1a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Guanylyl cyclase- activating protein 1, Guca1a ELISA KIT has been pre-coated with an Mouse Guanylyl cyclase-activating protein 1, Guca1a antibody specific to Mouse Guca1a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Guca1a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Guca1a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Guca1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Endocrinol 173(3): 467-474. 6. Zheng, X., L. Ying, et al. (2011). "Role of sulfhydryl-dependent dimerization of soluble guanylyl cyclase in relaxation of porcine coronary artery to nitric oxide." Cardiovasc Res 90(3): 565-572. 7. Tsai, E. J
-dependent protein kinase contributes to differential regulation and compartmentation of cGMP in rat cardiac myocytes." Circ Res 107(10): 1232-1240. 2. Guo, D., J. J. Zhang, et al. (2009). "Stimulation of guanylyl cyclase-D by bicarbonate
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
. CYC1p: minimal CYC1 promoter driving expression of the bait fusion proteins. SUC, SUC2 signal sequence derived from the yeast invertase protein; Cub, C‐terminal half of ubiquitin; LexA‐VP16, transcription factor cassette consisting of the E. coli LexA
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