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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Nuclear protein MDM1, Mdm1含量
- 适应物种:
小鼠
- 标记物:
Nuclear protein MDM1, Mdm1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Nuclear protein MDM1, Mdm1 ELISA KIT
Product Name:Mouse Nuclear protein MDM1, Mdm1 ELISA KIT
Packing:96T
Catalog No.:ELI-08463m
Gene Name:Mouse Mdm1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Mdm1
Alternative Name:Mouse Nuclear protein MDM1, Mdm1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nuclear protein MDM1, Mdm1 ELISA KIT allows for the in vitro quantitative determination of Mouse Mdm1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nuclear protein MDM1, Mdm1 ELISA KIT has been pre-coated with an Mouse Nuclear protein MDM1, Mdm1 antibody specific to Mouse Mdm1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mdm1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mdm1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mdm1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
in incomplete Freund’s adjuvant, the respective antisera were collected and assayed by enzyme-linked immunosorbent assay (ELISA) and purified by affinity chromatography on peptides conjugated to SulfoLink Coupling Gel (Pierce). Mouse brain proteins were prepared
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
two?hybrid system and its modifications have been successfully used over the past decade to investigate interactions between most classes of proteins expressed in a given cell or tissue. However, some proteins (e.g., integral membrane proteins or nuclear
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