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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Grainyhead-like protein 2 homolog, Grhl2含量
- 适应物种:
小鼠
- 标记物:
Grainyhead-like protein 2 homolog, Grhl2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Grainyhead- like protein 2 homolog, Grhl2 ELISA KIT
Product Name:Mouse Grainyhead- like protein 2 homolog, Grhl2 ELISA KIT
Packing:96T
Catalog No.:ELI-08295m
Gene Name:Mouse Grhl2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Grhl2
Alternative Name:Mouse Grainyhead-like protein 2 homolog, Grhl2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Grainyhead- like protein 2 homolog, Grhl2 ELISA KIT allows for the in vitro quantitative determination of Mouse Grhl2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Grainyhead- like protein 2 homolog, Grhl2 ELISA KIT has been pre-coated with an Mouse Grainyhead-like protein 2 homolog, Grhl2 antibody specific to Mouse Grhl2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Grhl2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Grhl2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Grhl2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
_004781 Homo sapiens vesicle-associated membrane protein 3 (cellubrevin) (VAMP3), mRNA 684 NM_003801 Homo sapiens GPAA1P anchor attachment protein 1 homolog (yeast) (GPAA1), mRNA 776 NM_004643 Homo sapiens poly(A) binding protein, nuclear 1 (PABPN1
Studies of the Ubiquitin Proteasome System
( protocol 33 ), or mouse E1 ( protocol 34 ) 100 mM HEPES, pH 7.5 10× thiolester buffer (see recipe ) [32 P]ubiquitin ( protocol 26 ) or 10 µg/µl “cold
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