ATP-dependent RNA helicase A,
Dhx9 ELISA KIT/ Mouse ATP-dependent RNA helicase A, Dhx9 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-08271m
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白ATP-dependent RNA helicase A, Dhx9含量
- 适应物种：
小鼠
- 标记物：
ATP-dependent RNA helicase A, Dhx9
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse ATP- dependent RNA helicase A, Dhx9 ELISA KIT
Product Name:Mouse ATP- dependent RNA helicase A, Dhx9 ELISA KIT
Packing:96T
Catalog No.:ELI-08271m
Gene Name:Mouse Dhx9
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Dhx9
Alternative Name:Mouse ATP-dependent RNA helicase A, Dhx9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse ATP- dependent RNA helicase A, Dhx9 ELISA KIT allows for the in vitro quantitative determination of Mouse Dhx9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse ATP- dependent RNA helicase A, Dhx9 ELISA KIT has been pre-coated with an Mouse ATP-dependent RNA helicase A, Dhx9 antibody specific to Mouse Dhx9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dhx9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dhx9 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dhx9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RNA Analysis by Nuclease Protection
Figure 5.1.2 Typical RNase protection assay using a probe for mouse β‐actin. A 300‐base mouse β‐actin probe was hybridized to two‐fold‐increasing amounts of mouse total‐liver RNA (from 0.625 to 10 µg; lanes 4 to 8) and analyzed by RNase protection
Linear amplification of limiting amounts of RNA for gene expression studies
Add 10µl mix to the RNA/primer sample and incubate at 42°C for 1 hour. Second strand synthesis (in RNAse-free PCR tubes) Prepare the following mix: 86µl DEPC-H2 O, 30µl 5x second strand buffer, 3µl 10mM dNTP mix, 8µl DNA Polymerase
to initiation factor 4A helicase hinder cap‐dependent translation by blocking ATP hydrolysis. RNA 9:394‐407. Ooi, A.T., Stains, C.I., Ghosh, I., and Segal, D.J. 2006. Sequence‐enabled
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