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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Glutathione peroxidase 3, Gpx3含量
- 适应物种:
小鼠
- 标记物:
Glutathione peroxidase 3, Gpx3
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Glutathione peroxidase 3, Gpx3 ELISA KIT
Product Name:Mouse Glutathione peroxidase 3, Gpx3 ELISA KIT
Packing:96T
Catalog No.:ELI-07761m
Gene Name:Mouse Gpx3
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Gpx3
Alternative Name:Mouse Glutathione peroxidase 3, Gpx3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Glutathione peroxidase 3, Gpx3 ELISA KIT allows for the in vitro quantitative determination of Mouse Gpx3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Glutathione peroxidase 3, Gpx3 ELISA KIT has been pre-coated with an Mouse Glutathione peroxidase 3, Gpx3 antibody specific to Mouse Gpx3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gpx3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gpx3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gpx3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA
Phospholipid hydroperoxide glutathione peroxidase (PHGPX or GPX4, E.C. 1.11.1.12) is one of the four identified selenium-dependent glutathione peroxidases (GPX) in mammals (1 ). Both of the pig (2.8 kb) and the mouse (4.0 kb) GPX4 genes
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Lacrimal Experimental Protocol (4/4/94)
5. Prepare 40 ml of EDTA/HBSS consisting of: 4 ml of 10 x HBSS, 8 ml of 10 x EDTA, and 28 ml of tissue culture sterile water. 6. Make up DMEM/gent. plus goodies as follows (for SFMOM, delete FBS, increase DMEM/gent. to 28.5 ml and add 30 µl of 10 µg/ml
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