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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Thymidine phosphorylase, Tymp含量
- 适应物种:
小鼠
- 标记物:
Thymidine phosphorylase, Tymp
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Thymidine phosphorylase, Tymp ELISA KIT
Product Name:Mouse Thymidine phosphorylase, Tymp ELISA KIT
Packing:96T
Catalog No.:ELI-07587m
Gene Name:Mouse Tymp
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Tymp
Alternative Name:Mouse Thymidine phosphorylase, Tymp
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Thymidine phosphorylase, Tymp ELISA KIT allows for the in vitro quantitative determination of Mouse Tymp concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Thymidine phosphorylase, Tymp ELISA KIT has been pre-coated with an Mouse Thymidine phosphorylase, Tymp antibody specific to Mouse Tymp .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tymp and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tymp , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tymp in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验We describe detailed methods to measure thymidine (dThd) and deoxyuridine (dUrd) concentrations and thymidine phosphorylase (TP) activity in biological samples. These protocols allow the detection of TP dysfunction in patients
Real‐Time Quantitative PCR Analysis of Mitochondrial DNA Content
array‐based comparative genomic hybridisation. Arch. Dis. Child 94:55‐58. Nishino, I., Spinazzola, A., and Hirano, M. 1999. Thymidine phosphorylase gene mutations in MNGIE
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