E3 ubiquitin-protein ligase HA

Mouse E3 ubiquitin- protein ligase HACE1, Hace1 ELISA KIT

Product Name:Mouse E3 ubiquitin- protein ligase HACE1, Hace1 ELISA KIT
Packing:96T

Catalog No.:ELI-07519m

Gene Name:Mouse Hace1

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse Hace1

Alternative Name:Mouse E3 ubiquitin-protein ligase HACE1, Hace1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse E3 ubiquitin- protein ligase HACE1, Hace1 ELISA KIT allows for the in vitro quantitative determination of Mouse Hace1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse E3 ubiquitin- protein ligase HACE1, Hace1 ELISA KIT has been pre-coated with an Mouse E3 ubiquitin-protein ligase HACE1, Hace1 antibody specific to Mouse Hace1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hace1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hace1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hace1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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