- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-07279m
- 2026年01月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Semaphorin-3E, Sema3e含量
- 适应物种：
小鼠
- 标记物：
Semaphorin-3E, Sema3e
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Semaphorin- 3E, Sema3e ELISA KIT
Product Name:Mouse Semaphorin- 3E, Sema3e ELISA KIT
Packing:96T
Catalog No.:ELI-07279m
Gene Name:Mouse Sema3e
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Sema3e
Alternative Name:Mouse Semaphorin-3E, Sema3e
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Semaphorin- 3E, Sema3e ELISA KIT allows for the in vitro quantitative determination of Mouse Sema3e concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Semaphorin- 3E, Sema3e ELISA KIT has been pre-coated with an Mouse Semaphorin-3E, Sema3e antibody specific to Mouse Sema3e .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sema3e and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sema3e , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sema3e in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
C3裂解产物 【参考范围】因检测方法不同而异，ELISA法正常为阴性；有文献报告＜12mg/L. 【影响因素】同血清Ig测定。 【临床意义】C3SP是补体在经典途径或旁路途径活化时，C3被C3转化酶等裂解而形成C3a和C3b两个具有重要生物学活性的片段。C3a是强碱性多肽，具有过敏毒素活性，可刺激组织肥大细胞和血液嗜碱性粒细胞释放组胺及其他生物学活性物质，增强毛细血管通透性并使血管平滑肌收缩。C3b不耐热，在血清中半衰期极短，具有一个不稳定结合位置和两个稳定
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