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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Hepatocyte nuclear factor 1-beta, Hnf1b含量
- 适应物种:
小鼠
- 标记物:
Hepatocyte nuclear factor 1-beta, Hnf1b
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Hepatocyte nuclear factor 1- beta, Hnf1b ELISA KIT
Product Name:Mouse Hepatocyte nuclear factor 1- beta, Hnf1b ELISA KIT
Packing:96T
Catalog No.:ELI-06911m
Gene Name:Mouse Hnf1b
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Hnf1b
Alternative Name:Mouse Hepatocyte nuclear factor 1-beta, Hnf1b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Hepatocyte nuclear factor 1- beta, Hnf1b ELISA KIT allows for the in vitro quantitative determination of Mouse Hnf1b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Hepatocyte nuclear factor 1- beta, Hnf1b ELISA KIT has been pre-coated with an Mouse Hepatocyte nuclear factor 1-beta, Hnf1b antibody specific to Mouse Hnf1b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hnf1b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hnf1b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hnf1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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