Hepatocyte nuclear factor 3-be

Mouse Hepatocyte nuclear factor 3- beta, Foxa2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

FOXA2,HNF3B; TCF3B; HNF-3-beta; HNF-3B; TCF-3B; forkhead box protein A2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3, beta; hepatocyte nuclear factor 3-beta; transcription factor 3B; forkhead box A2

Search name

Mouse FOXA2 ELISA KIT ,Mouse HNF3B ELISA KIT ,Mouse TCF3B ELISA KIT ,Mouse HNF-3-beta ELISA KIT ,Mouse HNF-3B ELISA KIT ,Mouse TCF-3B ELISA KIT ,Mouse forkhead box protein A2 ELISA KIT ,Mouse hepatic nuclear factor-3-beta ELISA KIT ,Mouse hepatocyte nuclear factor 3 ELISA KIT ,Mouse  beta ELISA KIT ,Mouse hepatocyte nuclear factor 3-beta ELISA KIT ,Mouse transcription factor 3B ELISA KIT ,Mouse forkhead box A2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Hepatocyte nuclear factor 3- beta, Foxa2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to FOXA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FOXA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FOXA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FOXA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.