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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Creatine kinase B-type, Ckb含量
- 适应物种:
小鼠
- 标记物:
Creatine kinase B-type, Ckb
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Creatine kinase B-type, Ckb ELISA试剂盒
Product Name: Mouse Creatine kinase B-type, Ckb ELISA试剂盒
Product Name: Mouse Creatine kinase B-type, Ckb ELISA试剂盒
Gene Name: Mouse Creatine kinase B-type, Ckb
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Creatine kinase B-type, Ckb
Alternative Name: Mouse Creatine kinase B-type, Ckb
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Creatine kinase B-type, Ckb ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Creatine kinase B-type, Ckb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Creatine kinase B-type, Ckb ELISA试剂盒 has been pre-coated with an Mouse Creatine kinase B-type, Ckb antibody specific to Mouse Creatine kinase B-type, Ckb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Creatine kinase B-type, Ckb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Creatine kinase B-type, Ckb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Creatine kinase B-type, Ckb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Molecular Investigation of LGMD2B-Haplotype Analysis and Mutation Screening
B have proximal muscle weakness with onset in the late teens, and highly elevated serum creatine kinase levels: The progression of the disease is generally slow. Muscle CT scanning in some patients indicates involvement of the distal muscles
, elevated serum creatine kinase and a necrotic-regeneration pattern on muscular biopsies (1 – 2 ). The study of these biopsies show that the integrity of the dystrophin-associated complex is preserved in these patients, because immunohistochemical staining
Analysis of Muscle Gene Transcription in Cultured Skeletal Muscle Cells
is key to understanding how different cells establish the muscle phenotype; it is also critical for developing methods to combat diseases such as muscular dystrophy. Using Muscle creatine kinase as a model, we outline the key steps involved
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