Mouse Complement component C8

Mouse Complement component C8 alpha chain, C8a ELISA KIT

Product Name:Mouse Complement component C8 alpha chain, C8a ELISA KIT
Packing:96T

Catalog No.:ELI-06322m

Gene Name:Mouse C8a

Detect Range:0.15-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse C8a

Alternative Name:Mouse Complement component C8 alpha chain, C8a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Complement component C8 alpha chain, C8a ELISA KIT allows for the in vitro quantitative determination of Mouse C8a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Complement component C8 alpha chain, C8a ELISA KIT has been pre-coated with an Mouse Complement component C8 alpha chain, C8a antibody specific to Mouse C8a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse C8a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse C8a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse C8a in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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