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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Interleukin-17F, Il17f含量
- 适应物种:
小鼠
- 标记物:
Interleukin-17F, Il17f
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Interleukin-17F, Il17f ELISA试剂盒
Product Name: Mouse Interleukin-17F, Il17f ELISA试剂盒
Product Name: Mouse Interleukin-17F, Il17f ELISA试剂盒
Gene Name: Mouse Interleukin-17F, Il17f
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Interleukin-17F, Il17f
Alternative Name: Mouse Interleukin-17F, Il17f
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Interleukin-17F, Il17f ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Interleukin-17F, Il17f concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Interleukin-17F, Il17f ELISA试剂盒 has been pre-coated with an Mouse Interleukin-17F, Il17f antibody specific to Mouse Interleukin-17F, Il17f .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Interleukin-17F, Il17f and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Interleukin-17F, Il17f , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Interleukin-17F, Il17f in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验ELISAs and Interleukin Research
ELISA (enzyme-linked immunosorbent assay) is a powerful, versatile, precise, and reliable quantitative technique for the measurement of antigens or antibodies in biologic samples. The ELISA technique is a widely used tool in biologic
Measurement of Interleukin-2 by Bioassay
immunosorbent assay (ELISA) methods, they are relatively easy to set up in a cell culture laboratory.
又是代谢的锅,不治之症银屑病终于有盼头了!上交王宏林教授团队Immunity重磅报道
,对 K5. Pp6fl/fl 鼠皮损处样本进行 scRNAseq 检测,发现 IL17a 和 IL17f 主要由 γδT 细胞表达 (图 N)。图片来源:Immunity PP6 通过激活 C/EBP-β 促进银屑病角质形成细胞 arg1 转录接下来,作者研究发现 K5.Pp6fl/fl 鼠中受损皮肤中磷酸化 C/EBPβ(p-C/EBPβ,Thr-188) 增强。值得关注的是,K5.Pp6fl/fl 鼠皮损处 C/EBP-β 靶基因水平增加。同时,在 PP6 缺陷鼠的原代表皮细胞和 siRNA 敲除
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