- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-05799m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Protein S100-A8, S100a8含量
- 适应物种：
小鼠
- 标记物：
Protein S100-A8, S100a8
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Protein S100-A8, S100a8 ELISA试剂盒
Product Name: Mouse Protein S100-A8, S100a8 ELISA试剂盒
Product Name: Mouse Protein S100-A8, S100a8 ELISA试剂盒
Gene Name: Mouse Protein S100-A8, S100a8
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Protein S100-A8, S100a8
Alternative Name: Mouse Protein S100-A8, S100a8
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Protein S100-A8, S100a8 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Protein S100-A8, S100a8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Protein S100-A8, S100a8 ELISA试剂盒 has been pre-coated with an Mouse Protein S100-A8, S100a8 antibody specific to Mouse Protein S100-A8, S100a8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Protein S100-A8, S100a8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Protein S100-A8, S100a8 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Protein S100-A8, S100a8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
Mapping Protein‐Protein Interactions with Phage‐Displayed Combinatorial Peptide Libraries
by purification of selected phage by ELISA. Alternatively, there is a bead?based affinity selection method. These methods allow one to readily isolate peptide ligands that bind to a protein target of interest and use the consensus sequence to search proteomic
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