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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Protein disulfide-isomerase A3, Pdia3含量
- 适应物种:
小鼠
- 标记物:
Protein disulfide-isomerase A3, Pdia3
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein disulfide-isomerase A3, Pdia3 ELISA试剂盒
Product Name: Mouse Protein disulfide-isomerase A3, Pdia3 ELISA试剂盒
Product Name: Mouse Protein disulfide-isomerase A3, Pdia3 ELISA试剂盒
Gene Name: Mouse Protein disulfide-isomerase A3, Pdia3
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Protein disulfide-isomerase A3, Pdia3
Alternative Name: Mouse Protein disulfide-isomerase A3, Pdia3
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Protein disulfide-isomerase A3, Pdia3 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Protein disulfide-isomerase A3, Pdia3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Protein disulfide-isomerase A3, Pdia3 ELISA试剂盒 has been pre-coated with an Mouse Protein disulfide-isomerase A3, Pdia3 antibody specific to Mouse Protein disulfide-isomerase A3, Pdia3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Protein disulfide-isomerase A3, Pdia3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Protein disulfide-isomerase A3, Pdia3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Protein disulfide-isomerase A3, Pdia3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Disulfide Conjugation of Peptides to Oligonucleotides and Their Analogs
of oligonucleotides and their analogs is being studied widely towards improving the delivery of oligonucleotides into cells. Amongst the many possible routes of conjugation, the disulfide linkage has proved to be the most popular. This reversible linkage
Methods to Detect Protein Glutathionylation
is a posttranslational modification that results in the formation of a mixed disulfide between glutathione and the thiol group of a protein cysteine residue. Glutathionylation of proteins occurs via both nonenzymatic mechanisms involving thiol/disulfide exchange
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
A3 amino acids of S. cerevisiae STE2 protein; Ost4, entire open reading frame of S. cerevisiae Ost4 protein; MCS, multiple cloning site. (E ) Multiple cloning sites for MbY2H vectors. View Image
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