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- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-04602m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Proto-oncogene Wnt-1, Wnt1含量
- 适应物种：
小鼠
- 标记物：
Proto-oncogene Wnt-1, Wnt1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Proto-oncogene Wnt-1, Wnt1 ELISA试剂盒
Product Name: Mouse Proto-oncogene Wnt-1, Wnt1 ELISA试剂盒
Product Name: Mouse Proto-oncogene Wnt-1, Wnt1 ELISA试剂盒
Gene Name: Mouse Proto-oncogene Wnt-1, Wnt1
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Proto-oncogene Wnt-1, Wnt1
Alternative Name: Mouse Proto-oncogene Wnt-1, Wnt1
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Proto-oncogene Wnt-1, Wnt1 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Proto-oncogene Wnt-1, Wnt1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Proto-oncogene Wnt-1, Wnt1 ELISA试剂盒 has been pre-coated with an Mouse Proto-oncogene Wnt-1, Wnt1 antibody specific to Mouse Proto-oncogene Wnt-1, Wnt1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Proto-oncogene Wnt-1, Wnt1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Proto-oncogene Wnt-1, Wnt1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Proto-oncogene Wnt-1, Wnt1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验v468.chapter 1 经典 Wnt/b-Catenin 信号通路 （英文完整版）
, b-catenin , Groucho , Tcf , Target gene , Constitutive activation , Stem cell ,Colon cancer. 1. Introduction The Wnt pathway derives its name from the Drosophila (fruit-fly)W ingless gene and the mouse I NT -1 gene. The mouse Wnt1gene
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
phosphate-buffered saline (PBS) containing 0.1% (w/v)bovine serum albumin (BSA) at a concentration of 50 μg/mLand stored in aliquots at –20°C (see Note 2 ). 3. Mouse L cells that express and secrete Wnt3a (ATCC, Manassas,VA; #CRL-2647) and the control L
transgene with a focus on tracking cardiac neural crest cells using the Wnt1-Cre and R26R mouse lines. We also provide a protocol using fluorescence-activated cell sorting for collecting neural crest cells for further analysis. These protocols can be used
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