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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Adenylate cyclase type 1, Adcy1含量
- 适应物种:
小鼠
- 标记物:
Adenylate cyclase type 1, Adcy1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Adenylate cyclase type 1, Adcy1 ELISA试剂盒
Product Name: Mouse Adenylate cyclase type 1, Adcy1 ELISA试剂盒
Product Name: Mouse Adenylate cyclase type 1, Adcy1 ELISA试剂盒
Gene Name: Mouse Adenylate cyclase type 1, Adcy1
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Adenylate cyclase type 1, Adcy1
Alternative Name: Mouse Adenylate cyclase type 1, Adcy1
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Adenylate cyclase type 1, Adcy1 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Adenylate cyclase type 1, Adcy1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Adenylate cyclase type 1, Adcy1 ELISA试剂盒 has been pre-coated with an Mouse Adenylate cyclase type 1, Adcy1 antibody specific to Mouse Adenylate cyclase type 1, Adcy1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Adenylate cyclase type 1, Adcy1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Adenylate cyclase type 1, Adcy1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Adenylate cyclase type 1, Adcy1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验-dependent calcium channels, AT1 receptors can couple to inhibition of adenylate cyclase via the guanine nucleotide binding protein Gi. Beside acting directly through Gi , AT1 receptors can modulate levels of cyclic AMP (cAMP) indirectly through receptor
in a variety of signaling pathways. ADP-ribosylation not only uncouples these G-proteins from their receptors but in addition also deranges the adenylate cyclase system and in this way interferes with second-messenger pathways (3 –7 ). The modification
Fluorescent In Situ Transcription in Cells and Tissues
sequence (Monsma et al., ). (A ) Transcript staining is evident as bright fluorescent neurons (arrows) of medium diameter in this 10‐µm‐thick frozen/fixed tissue section of the wild‐type (WT) mouse striatum. (B ) Fluorescence is diminished in the mutant
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