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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Fatty acid-binding protein, heart, Fabp3含量
- 适应物种:
小鼠
- 标记物:
Fatty acid-binding protein, heart, Fabp3
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Fatty acid-binding protein, heart, Fabp3 ELISA试剂盒
Product Name: Mouse Fatty acid-binding protein, heart, Fabp3 ELISA试剂盒
Product Name: Mouse Fatty acid-binding protein, heart, Fabp3 ELISA试剂盒
Gene Name: Mouse Fatty acid-binding protein, heart, Fabp3
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Fatty acid-binding protein, heart, Fabp3
Alternative Name: Mouse Fatty acid-binding protein, heart, Fabp3
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Fatty acid-binding protein, heart, Fabp3 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Fatty acid-binding protein, heart, Fabp3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Fatty acid-binding protein, heart, Fabp3 ELISA试剂盒 has been pre-coated with an Mouse Fatty acid-binding protein, heart, Fabp3 antibody specific to Mouse Fatty acid-binding protein, heart, Fabp3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Fatty acid-binding protein, heart, Fabp3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Fatty acid-binding protein, heart, Fabp3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Fatty acid-binding protein, heart, Fabp3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验the concentration of unbound FA is a method using the fluorescent-(acrylodan) labeled intestinal FA-binding protein (ADIFAB). Under appropriate conditions, ADIFAB fluorescence measures unbound FA, regardless of any third phase such as albumin, FA-binding proteins
Mammalian Fatty Acid Elongases
have been known for many years, recent advances have revealed a family of enzymes involved in the first step of the reaction, i.e., the condensation reaction. Seven fatty acid elongase subtypes (Elovl #1–7) have been identified in the mouse, rat, and human genomes
Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
The authors’ laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays
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