RNA-binding protein 10, Rbm10
ELISA KIT/ Mouse RNA-binding protein 10, Rbm10 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-52313m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
RNA-binding protein 10, Rbm10
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse RNA- binding protein 10, Rbm10 ELISA KIT
Product Name:Mouse RNA- binding protein 10, Rbm10 ELISA KIT
Packing:96T
Catalog No.:ELI-52313m
Gene Name:Mouse Rbm10
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Rbm10
Alternative Name:Mouse RNA-binding protein 10, Rbm10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse RNA- binding protein 10, Rbm10 ELISA KIT allows for the in vitro quantitative determination of Mouse Rbm10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse RNA- binding protein 10, Rbm10 ELISA KIT has been pre-coated with an Mouse RNA-binding protein 10, Rbm10 antibody specific to Mouse Rbm10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rbm10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rbm10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rbm10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. Vortex the tube for 30 seconds. 18. Centrifuge at maximum speed ($13000 x g) at 4°C for 3 minutes. Aspirate the supernatant and air dry the RNA pellet by inverting the tube on a absorbent paper for 5-10 minutes 19. Add 50-100μl of DEPC
DNase I Footprint Analysis of Protein‐DNA Binding
I (DNase I) protection mapping, or footprinting, is a valuable technique for locating the specific binding sites of proteins on DNA. The basis of this assay is that bound protein protects the phosphodiester backbone of DNA from DNase I?catalyzed hydrolysis
RNA AND PROTEIN EXTRACTION FROM THE SAME SAMPLE
of homogenate) 5. Centrifuge at 12,000 xg for 20 min (4o C). Pour the supernatant into a 15 ml tube 6. Precipitated RNA with LiCl by adding 1/4 vol. (1.1 mL) of 10 M LiCl, (a white precipitate appears) mix gently. Place tube
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