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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Leukotriene-B(4) omega-hydroxylase 2, Cyp4f3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Leukotriene- B(4) omega- hydroxylase 2, Cyp4f3 ELISA KIT
Product Name:Mouse Leukotriene- B(4) omega- hydroxylase 2, Cyp4f3 ELISA KIT
Packing:96T
Catalog No.:ELI-31958m
Gene Name:Mouse Cyp4f3
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cyp4f3
Alternative Name:Mouse Leukotriene-B(4) omega-hydroxylase 2, Cyp4f3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Leukotriene- B(4) omega- hydroxylase 2, Cyp4f3 ELISA KIT allows for the in vitro quantitative determination of Mouse Cyp4f3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Leukotriene- B(4) omega- hydroxylase 2, Cyp4f3 ELISA KIT has been pre-coated with an Mouse Leukotriene-B(4) omega-hydroxylase 2, Cyp4f3 antibody specific to Mouse Cyp4f3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cyp4f3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cyp4f3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cyp4f3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
Delayre‐Orthez, C., Becker, J., de Blay, F., Frossard, N., and Pons, F. 2005b. Exposure to endotoxins during sensitization prevents further endotoxin‐induced exacerbation of airway inflammation in a mouse model of allergic asthma. Int. Arch
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
culture and preparation of tissue sections. Obviously, utilizing commercial kits the duration of method is reduced (2-3 hours for immunoenzymatic kit and 1-2 hours for fluorescein kit). B.3.4 Key references 1. Gavrieli, Y., Sherman
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