- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-03801m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Serine含量
- 适应物种：
小鼠
- 标记物：
Serine
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Serine/arginine- rich splicing factor 3, Srsf3 ELISA KIT
Product Name:Mouse Serine/arginine- rich splicing factor 3, Srsf3 ELISA KIT
Packing:96T
Catalog No.:ELI-03801m
Gene Name:Mouse Srsf3
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Srsf3
Alternative Name:Mouse Serine/arginine-rich splicing factor 3, Srsf3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine/arginine- rich splicing factor 3, Srsf3 ELISA KIT allows for the in vitro quantitative determination of Mouse Srsf3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Serine/arginine- rich splicing factor 3, Srsf3 ELISA KIT has been pre-coated with an Mouse Serine/arginine-rich splicing factor 3, Srsf3 antibody specific to Mouse Srsf3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Srsf3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Srsf3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Srsf3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验较高纯度的包涵体。 2.包涵体溶解要彻底，一般应使溶解液体量较大，不要怕蛋白被稀释，要有助溶措施。 3.透析前后均要离心 4.透析不能太快. 5.纯化的最佳条件要摸索。 小技巧： 1）包含体要彻底洗涤干净，洗涤液中加入适量Triton-X100. 2) 包含体溶解后装入透析袋在复性液中复性 3）复性液的组成很有讲究，本人使用 Oxidised Glutathione/ Reduced Glutathione 还加入一定的L-Arginine-Hydrochloride 12-24
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