- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-03689m
- 2026年05月21日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
源叶生物 十万种现货试剂速发，品质稳、品类全，实验不再等！Takara 秋促来袭，提取+反转录+定量套装仅 1200 元abinScience 体内级抗体 全年锁价 低至 ￥1200阿拉丁 22万+ 科研级产品现货速发，品质严控、科研无忧- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Sphingosine-1-phosphate phosphatase 1, Sgpp1含量
- 适应物种：
小鼠
- 标记物：
Sphingosine-1-phosphate phosphatase 1, Sgpp1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Sphingosine- 1- phosphate phosphatase 1, Sgpp1 ELISA KIT
Product Name:Mouse Sphingosine- 1- phosphate phosphatase 1, Sgpp1 ELISA KIT
Packing:96T
Catalog No.:ELI-03689m
Gene Name:Mouse Sgpp1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Sgpp1
Alternative Name:Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Sphingosine- 1- phosphate phosphatase 1, Sgpp1 ELISA KIT allows for the in vitro quantitative determination of Mouse Sgpp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Sphingosine- 1- phosphate phosphatase 1, Sgpp1 ELISA KIT has been pre-coated with an Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 antibody specific to Mouse Sgpp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sgpp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sgpp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sgpp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
：
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验4) 0.87 gSodium phosphate monobasic (Na2HPO4* H2O) 0.54 gSodium chloride 8.5 gHPLC water 1.00 LAdgust PH to 7.4 with 1N NaOH 1M HClSLGMA Immunp Chemical SIGMA DiagnosticsAnti-mouse IgG (Fab specific) Sigma 104 PhosphataseAlaline Phosphatase conjugate
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase-conjugated antibodies. However, by carefully controlling the reaction time in X-gal so that only a small amount of indigo is produced (Vaysse and Goldman, 1990), by having ß-gal localized to the nucleus when the cellular antigen is non-nuclear
ELISA PROTOCOL 【Yale University 】
-nitrophenyl phosphate (5 mg/tablet). SigmaCat.# N93895) AP-conjugated secondary Ab: AP-Rabbit anti-mouse IgG(Zymed). Reconstitute in ddH2O as prescribed by manufacturer andaliquot and store at -70 °C.6) 100 mM EDTA pH
技术资料暂无技术资料 索取技术资料
