Sphingosine-1-phosphate phosph
atase 1, Sgpp1 ELISA KIT/ Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-03689m
- 2026年01月02日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Sphingosine-1-phosphate phosphatase 1, Sgpp1含量
- 适应物种：
小鼠
- 标记物：
Sphingosine-1-phosphate phosphatase 1, Sgpp1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 ELISA试剂盒
Product Name: Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 ELISA试剂盒
Product Name: Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 ELISA试剂盒
Gene Name: Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1
Alternative Name: Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 ELISA试剂盒 has been pre-coated with an Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 antibody specific to Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sphingosine-1-phosphate phosphatase 1, Sgpp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
ELISA PROTOCOL 【Yale University 】
-nitrophenyl phosphate (5 mg/tablet). SigmaCat.# N93895) AP-conjugated secondary Ab: AP-Rabbit anti-mouse IgG(Zymed). Reconstitute in ddH2O as prescribed by manufacturer andaliquot and store at -70 °C.6) 100 mM EDTA pH
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