- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-03545m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Prolyl 4-hydroxylase subunit alpha-1, P4ha1含量
- 适应物种：
小鼠
- 标记物：
Prolyl 4-hydroxylase subunit alpha-1, P4ha1
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Prolyl 4- hydroxylase subunit alpha- 1, P4HA1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
P4HA1,P4HA; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline, 2-oxoglutarate 4-dioxygenase ,proline 4-hydroxylase alpha polypeptide I; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; prolyl 4-hydroxylase alpha polypeptide I; prolyl 4-hydroxylase subunit alpha 1, Prolyl 4-hydroxylase subunit alpha-1,4-PH alpha-1
Search name
Mouse P4HA1 ELISA KIT ,Mouse P4HA ELISA KIT ,Mouse C-P4Halpha(I) ELISA KIT ,Mouse collagen prolyl 4-hydroxylase alpha(I) ELISA KIT ,Mouse procollagen-proline ELISA KIT ,Mouse 2-oxoglutarate 4-dioxygenase ELISA KIT ,Mouse proline 4-hydroxylase alpha polypeptide I ELISA KIT ,Mouse procollagen-proline ELISA KIT ,Mouse 2-oxoglutarate-4-dioxygenase subunit alpha-1 ELISA KIT ,Mouse prolyl 4-hydroxylase alpha polypeptide I ELISA KIT ,Mouse prolyl 4-hydroxylase subunit alpha 1 ELISA KIT ,Mouse Prolyl 4-hydroxylase subunit alpha-1 ELISA KIT ,Mouse 4-PH alpha-1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse 4-PH alpha-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-PH alpha-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 4-PH alpha-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 4-PH alpha-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-PH alpha-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
Molecular Imaging of RNA Interference Therapy Targeting PHD2 for Treatment of Myocardial Ischemia
of hypoxia inducible factor-1 alpha (HIF-1α) transcriptional factor can activate several downstream angiogenic genes. However, HIF-1α is naturally degraded by prolyl hydroxylase-2 (PHD2) protein. Recently, we cloned the mouse PHD2 gene by comparing
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