Ubiquitin-conjugating enzyme E
2 B, Ube2b ELISA KIT/ Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-03458m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Ubiquitin-conjugating enzyme E2 B, Ube2b含量
- 适应物种：
小鼠
- 标记物：
Ubiquitin-conjugating enzyme E2 B, Ube2b
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b ELISA试剂盒
Product Name: Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b ELISA试剂盒
Product Name: Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b ELISA试剂盒
Gene Name: Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b
Alternative Name: Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b ELISA试剂盒 has been pre-coated with an Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b antibody specific to Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ubiquitin-conjugating enzyme E2 B, Ube2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
NF-~B激活中抑制成分I-~cB的降解，以及免疫调节一章中将提到细胞因子信号转导抑制蛋白(SOCS)对底物的作用，皆涉及这一泛素―蛋白酶体途径。 蛋白质泛素化系统由3个组分构成，一个称为泛素激活酶n，它可利用水解ATP释放的能量以其胱氨酸残基(Cys)的巯基与泛素C端的甘氨酸残基(Gly)形成高能硫酯键。然后连接在殿上的泛素被转移到另一个泛素结合酶E2上，同时，被选中的靶蛋白与第三个组分即靶蛋白泛素连接酶E3结合。E2然后将与其连接的泛素转移到靶蛋白上，并与靶蛋白赖氨酸残基(Lys)―NH
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