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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Heat shock protein beta-6, Hspb6含量
- 适应物种:
小鼠
- 标记物:
Heat shock protein beta-6, Hspb6
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Heat shock protein beta-6, Hspb6 ELISA试剂盒
Product Name: Mouse Heat shock protein beta-6, Hspb6 ELISA试剂盒
Product Name: Mouse Heat shock protein beta-6, Hspb6 ELISA试剂盒
Gene Name: Mouse Heat shock protein beta-6, Hspb6
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Heat shock protein beta-6, Hspb6
Alternative Name: Mouse Heat shock protein beta-6, Hspb6
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Heat shock protein beta-6, Hspb6 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Heat shock protein beta-6, Hspb6 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Heat shock protein beta-6, Hspb6 ELISA试剂盒 has been pre-coated with an Mouse Heat shock protein beta-6, Hspb6 antibody specific to Mouse Heat shock protein beta-6, Hspb6 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Heat shock protein beta-6, Hspb6 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Heat shock protein beta-6, Hspb6 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Heat shock protein beta-6, Hspb6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Isolation of Heat Shock Protein Complexes
Heat shock proteins (Hsp) are molecular chaperones with the capability to interact with a wide range of other proteins and are thus often found coupled with other heat shock and non-heat shock proteins. This can be an advantage to study
指将生物的整体、组织、细胞等从其生活的温度范围内急剧地从低温移向高温时,可显著地促进合成的一组蛋白质。例如将果蝇的幼虫或培养细胞从28℃移至 35℃时,则几乎大部分的蛋白质合成停止;与此相反,而休克蛋白的合成却反而被促进。这种促进作用主要是在转录 DNA的合成(转录)阶段产生的。同样的现象也见于哺乳类动物、培养细胞、原生动物、植物组织和细菌等。另外观察到,由休克以外的其他处理也会发生类似的现象。这种现象的生理意义尚不清楚,但推测是与生物的温度适应现象有关系。
Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types
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