- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-02682m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白SPARC, Sparc含量
- 适应物种：
小鼠
- 标记物：
SPARC, Sparc
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse SPARC, Sparc ELISA试剂盒
Product Name: Mouse SPARC, Sparc ELISA试剂盒
Product Name: Mouse SPARC, Sparc ELISA试剂盒
Gene Name: Mouse SPARC, Sparc
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse SPARC, Sparc
Alternative Name: Mouse SPARC, Sparc
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse SPARC, Sparc ELISA试剂盒 allows for the in vitro quantitative determination of Mouse SPARC, Sparc concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse SPARC, Sparc ELISA试剂盒 has been pre-coated with an Mouse SPARC, Sparc antibody specific to Mouse SPARC, Sparc .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse SPARC, Sparc and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse SPARC, Sparc , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse SPARC, Sparc in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Circulation：华科协和医院程翔等报道组织特异性Treg 为基础的心脏免疫治疗新思路
的机制 为进一步阐明心肌梗死后心脏 Tregs 促进组织修复的机制，研究人员对心脏 Tregs 的转录组数据进行分析，发现 Sparc 是心脏高表达差异基因中最显著的差异基因之一，实时定量 PCR 结果也证实了心脏 Sparc 的高转录水平。作为一种胶原结合母细胞蛋白，Sparc 在胶原组装成细胞外基质 (ECM) 中起关键作用，已有报道其在心肌梗死后的心室完整性保护中起关键作用。 图片来源：Circulation为了从功能上确定 Sparc 是否在心肌梗死后心脏 Tregs 介导的组织修复中
Protein detection onto PVDF membranes
[3]. Scanning The Laser Densitometer (4000 x 5000 pixels; 12 bits/pixel) from Molecular Dynamics and the GS-700 from Bio-Rad have been used as scanning device. This scanners were linked to Sparc workstations and Macintosh computers.
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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