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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白soluble platelet-selectin, sP-selectin含量
- 适应物种:
小鼠
- 标记物:
soluble platelet-selectin, sP-selectin
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
GMP-140
Product Name: GMP-140
Product Name: GMP-140
Gene Name: GMP-140
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: GMP-140
Alternative Name: GMP-140
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: GMP-140 allows for the in vitro quantitative determination of GMP-140 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in GMP-140 has been pre-coated with an GMP-140 antibody specific to GMP-140 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GMP-140 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GMP-140, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of GMP-140 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验2-4 selectin分子的结构模式图 表2-3 selectin 家族的组成、分布及其相应配体 selectin家族成员 分布 分子量(kDa) 配体 L-selectin(CD62L,LECAM-1) 白细胞 75~80 PNAd LAM Mel14(小鼠) S-Lewis x P-selectin 血管内皮细胞,血小板 140 S-Lewis x (CD62P,GMP-140,PADGEM) (凝血酶、组胺、白三烯刺激后从α颗粒内与质膜融合而表达
selectin,LAM- 1,LECAM- 1) 主要表达细胞: T,B,M,NK,PMN,Eo[AS] 分子质量(kDa)和结构: gp76(C- lectin SF,含EGF样结构域和2个CCP结构域) 功 能: 粘附CD15s、E- selectin,P- selectin,结合Gly- CAM- 1、CD34、MAd- CAM- 1上的O连接糖 CD62P 常用单克隆抗体或代号: G2,AK- 6;(P- selectin,GMP- 140
OUTLINE We consider manual counts as less quick but more precize than authomatic (electronic) since in the latter case the counts are based on the size of particles, so everything that has the same size as platelet will be counted
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