Mouse enkephalin ELISA试剂盒

Mouse enkephalin, ENK ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

Mouse ENK ELISA KIT ,Mouse enkephalin ELISA KIT ,Mouse PENK ELISA KIT ,Mouse enkephalin A ELISA KIT ,Mouse PENK-A ELISA KIT ,Mouse preproenkephalin ELISA KIT ,Mouse proenkephalin ELISA KIT

Intended use

Mouse ENK ELISA kit allows for the in vitro quantitative determination of Mouse enkephalin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse ENK ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ENK, During the reaction, ENK in the sample or standard competes with a fixed amount of biotin-labeled ENK for sites on a pre-coated Monoclonal antibody specific to ENK. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ENK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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