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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA含量
- 适应物种:
小鼠
- 标记物:
Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA
Product Name: Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA
Product Name: Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA
Gene Name: Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA
Alternative Name: Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA allows for the in vitro quantitative determination of Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA has been pre-coated with an Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA antibody specific to Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Soluble Intercellular Adhesion Molecule-1, sICAM-1 ELISA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验组。 3. 可溶性细胞间粘附分子 5 细胞间粘附分子 5(Intercellular adhesion molecule-5,ICAM5)是一种中枢神经系统来源的抗炎蛋白,它是一种神经元糖蛋白,在脑中表达,也可存在于血液中,经由T细胞移动性和趋化作用的抑制而作为抗炎蛋白发挥功能。 可溶性细胞间粘附分子(soluble Intercellular adhesion molecule-5,sICAM5)可以作为帮助我们诊断癫痫的生物标志物。在癫痫患者血液中,sICAM-5浓度降低,而促进炎症的白细胞
【申请上传】Current Protocols in Immunology
Immunocompromised Animals Unit 1.3 Handling and Restraint Unit 1.4 Anesthesia Unit 1.5 Animal Identification Section II Use of Mouse, Rat, Hamster, and Rabbit Unit 1.6 Parenteral Injections Unit 1.7 Blood Collection Unit 1.8 Euthanasia Section III Selected
, either by culturing cells with radioactive precursor or by labeling the molecule after synthesis has been completed (e.g., by radioiodination to iodinate tyrosine residues or by sodium [3H]borohydride reduction to label carbohydrate). Having a radioactive antigen
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