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- 详细信息
- 文献和实验
- 技术资料
- 库存:
231
- 英文名:
N-Acetylneuraminic acid aldolase
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
-20℃
- 规格:
1KU
特别提示:包括N-乙酰神经氨酸醛缩酶在内,本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
产品名称:N-乙酰神经氨酸醛缩酶
英文名称:N-Acetylneuraminic acid aldolase
产品货号:Y16189
产品规格:1KU
CAS:9027-60-5
介绍:One unit will convert one micromole of NAcetylneuraminate to N-Acetyl-Dmannosamine per minute at pH 7.5 at 37℃
储存条件:-20℃
来源:微生物
外观:粉末
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文献和实验Primer Premier: Program for Design of Degenerate Primers from a Protein Sequence
of novel genes are often faced with the problem that only a partial protein sequence is known. Several procedures are commonly used involving the reverse translation of the protein sequence into a DNA sequence. These include synthesis of a short
.5 60 1500 420 4 26 30 80
6xHis-tagged protein purification using Qiagen Ni-NTA Column
for 15’ on ice and resuspend the cells in lysis buffer at 2-5 ml per gram weight (I use 10ml for 500ml culture)2.Add lysozyme to 1mg/ml and 1xprotease inhibitors and incubate on ice for 30’3.Sonicate on ice (use 6x10” bursts with a 10” cooling period
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